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KMID : 1094020170340020070
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Journal of Veterinary Clinics
2017 Volume.34 No. 2 p.70 ~ p.75
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Fucoidan Upregulates Chemotactic Activity of Porcine Peripheral Blood Polymorphonuclear Cells to Interleukin-8 by PI3K Activation
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Kang Song-Ai
Ahn Chang-Hwan
Kang Byeong-Teck
Kang Ji-Houn
Jeung Eui-Bae
Yang Mhan-Pyo
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Abstract
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Fucoidan increases the chemotactic activity of peripheral blood polymorphonuclear cells (PMNs) through interleukin (IL)-8 produced by peripheral blood mononuclear cells (PBMCs). It has been demonstrated that fucoidan can regulate the chemotaxis of PMNs by activating F-actin polymerization. The objectives of this study are to investigate the direct effect of fucoidan on the chemotaxis of porcine PMNs and to examine whether this effect is associated with changes in phosphoinositide 3-kinase (PI3K) activity. The chemotactic activity of porcine PMNs was evaluated by modified Boyden chamber assay. Akt phosphorylation activity, a main downstream of PI3K, was measured by Western blotting assay. Fucoidan itself has no chemoattractant effect for PMNs. However, direct treatment of PMNs with fucoidan showed higher chemotactic activity to porcine recombinant (pr) IL-8 than that of PMNs without fucoidan. The increased chemotactic activity of fucoidan-treated PMNs to pr IL-8 was suppressed by treatment of wortmannin, an inhibitor of PI3K. Treatment of PMNs with fucoidan also increased Akt phosphorylation level. This increase was also suppressed by wortmannin. These results suggested that fucoidan can upregulate chemotactic activity of porcine PMNs to IL-8, which is associated with PI3K activation.
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KEYWORD
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chemotaxis , fucoidan , PI3K , Akt , PMNs , porcine , IL-8
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